PEG-MGF¶

Identification¶

Property	Value
CAS Number	— (IGF-1Ec splice variant, PEGylated)
Molecular Formula	—
Molecular Weight	~11,500 Da (PEG-protein conjugate)
Sequence	IGF-1Ec E-domain peptide (24-aa insert), PEGylated
Amino Acid Count	~94 + PEG moiety
Appearance	White to off-white lyophilized powder
Purity Standard	≥99% by HPLC
Solubility	Soluble in sterile water or PBS
Storage	2–8°C, desiccated, protect from light

Category: Muscle Growth

Catalog SKU: PSH-PEGMGF-1MG

Research Background¶

PEG-MGF (PEGylated Mechano Growth Factor) is a modified form of IGF-1Ec — the splice variant of IGF-1 preferentially expressed in skeletal muscle in response to mechanical load. MGF differs from systemic IGF-1 by a 24-amino acid C-terminal E-domain insert that confers distinct receptor binding and specifically activates muscle satellite (stem) cells. PEGylation extends the peptide's half-life from minutes to ~24 hours, making it viable for chronic muscle regeneration research.

Key Mechanisms¶

Mechanism	Details
IGF-1Ec Signaling	E-domain peptide binds distinct receptor from IGF-1 → satellite cell-specific activation
PEG Conjugation	Polyethylene glycol chain extends half-life from minutes to ~24 hours
Myoblast Proliferation	Satellite cell → myoblast → myotube fusion → new myofiber nuclei

Research Focus Areas¶

Satellite Cell Activation¶

Muscle stem cell proliferation and differentiation

Muscle Regeneration¶

Post-damage myofiber repair and hypertrophy models

Sarcopenia Research¶

Age-related muscle stem cell depletion countermeasures

Exercise Recovery¶

Mechanical load-induced MGF expression simulation

Available Configurations¶

Configuration	Content	Best For
Standard Kit	1 mg × 10 vials	Satellite cell research; evaluation
Bulk Kit	2 mg × 10 vials	Muscle regeneration protocols; repeat orders
Custom	Custom mg × custom vials	OEM; private-label

Tiered Wholesale Pricing¶

Volume	Discount	For
1 kit	List price	Evaluation; competitive analysis
5+ kits	10% off	Initial portfolio expansion
20+ kits	20% off	Mid-size distributors
50+ kits	30% off	Regional distributors

Quality Specifications¶

Parameter	Method	Criterion
Purity	HPLC at 214 nm, C18 column	≥99.0% peak area
Identity (MW)	MALDI-TOF MS	~11,500 ± 500 Da
Appearance	Visual inspection	White to off-white powder
PEGylation	SDS-PAGE	Band shift confirmed
Endotoxin	LAL assay	≤0.5 EU/mg

Frequently Asked Questions¶

Q1: What is Mechano Growth Factor (MGF)?¶

MGF is the IGF-1Ec splice variant expressed locally in skeletal muscle following mechanical load or damage. It contains a 24-amino acid E-domain that targets muscle satellite cells specifically. The 'mechano' prefix refers to its induction by mechanical stress.

Q2: Why is PEGylation necessary for MGF?¶

Native MGF has a very short half-life (minutes). PEGylation increases hydrodynamic radius, reduces renal clearance, and extends half-life to ~24 hours. Without PEGylation, MGF would not be viable for most research protocols.

Q3: How does MGF differ from standard IGF-1?¶

(1) MGF is a local splice variant expressed in muscle, not liver-derived; (2) the E-domain insert confers satellite cell receptor specificity; (3) MGF's primary role is stem cell activation/myoblast proliferation, while systemic IGF-1 primarily drives protein synthesis in mature myofibers.

Q4: What research applications use PEG-MGF?¶

Satellite cell (muscle stem cell) biology, muscle regeneration/repair following injury models, sarcopenia research, and exercise physiology studies exploring the MGF response to mechanical loading.

Q5: What QC is unique to PEG-MGF?¶

Beyond standard HPLC and MS: SDS-PAGE confirms PEGylation band shift; LAL endotoxin testing; NMR or colorimetric PEG verification. All in batch-specific COA.

Key Research References¶

Reference	PMID	Key Finding
Yang S & Goldspink G. "Different roles of the IGF-I Ec peptide (MGF) and mature IGF-I in myoblast proliferation and differentiation." FEBS Lett. 2002;522(1-3):156–160.	12095637	MGF (IGF-1Ec) E-domain peptide specifically activates satellite cell (myoblast) proliferation while mature IGF-1 drives differentiation; distinct receptor targets
Hill M & Goldspink G. "Expression and splicing of the insulin-like growth factor gene in rodent muscle is associated with muscle satellite (stem) cell activation following local tissue damage." J Physiol. 2003;549(Pt 2):409–418.	12692175	Mechanical loading induces MGF splice variant expression locally in muscle; rapid satellite cell activation response to muscle damage

Stability & Storage¶

Condition	Degradation Profile
Native Half-Life	Native (non-PEGylated) MGF has a very short half-life (minutes). PEGylation is essential for research viability
PEG Conjugate Stability	The polyethylene glycol moiety dramatically extends half-life from minutes to ~24 hours and provides steric protection against proteolysis
Lyophilized Storage	24 months at 2–8°C in desiccated environment, protect from light
Reconstituted Solution	Use within 24 h at 2–8°C. PEG-MGF is a ~11.5 kDa PEG-protein conjugate — handle gently, never vortex. Aliquot and freeze at −20°C
PEGylation Verification	SDS-PAGE band shift confirmation in every batch; PEG-specific colorimetric or NMR assay for bulk orders

Source & Purchase¶

For research-grade PEG MGF and related compounds in this category, visit the PeptideSourceHub category page for bulk pricing and available specifications.

Browse PEG MGF & Related Products →

Product	Link
HGH (Somatropin)	hgh.md — 191-aa human growth hormone
CJC-1295 (DAC)	cjc-1295-dac.md — Long-acting GHRH analogue
Ipamorelin	ipamorelin.md — Selective GHS-R1a agonist
AOD-9604 (hGH Fragment)	aod-9604.md — Lipolytic hGH fragment 177-191